# Meltzerlab GEO Microarray Tool - View GSE Details
 
Meltzerlab | GEO Site Home | GSE-GPL-GSM | GPL | GSE | GSM | GDS | GDS Subset | sMatrix | Help

View GSE Details

Previous Record - Next Record - Jump to ID:
 
 
ID: 21
Geo Accession: GSE21 (GEO Site)
Title: snf/swi mutants of S. cerevisiae.
Platform id: GPL57 GPL65 GPL67
Sample id:
Total 12 GSM
Status: Public on Feb 12 2002
Submission date: 2002-02-08
Last update date: 2013-01-18
Pubmed id: 10725359  
Summary: The Saccharomyces cerevisiae Snf/Swi complex has been previously demonstrated to control transcription and chromatin structure of particular genes in vivo and to remodel nucleosomes in vitro. We have performed whole-genome expression analysis, using DNA microarrays, to study mutants deleted for a gene encoding one conserved (Snf2) or one unconserved (Swi1) Snf/Swi component. This analysis was performed on cells grown in both rich and minimal media. The microarray results, combined with Northern blot, computational, and genetic analyses, show that snf2Delta and swi1Delta mutations cause similar effects on mRNA levels, that Snf/Swi controls some genes differently in rich and minimal media, and that Snf/Swi control is exerted at the level of individual genes rather than over larger chromosomal domains. In addition, this work shows that Snf/Swi controls mRNA levels of MATalpha-specific genes, likely via controlling transcription of the regulators MATalpha1 and MCM1. Finally, we provide evidence that Snf/Swi acts both as an activator and as a repressor of transcription, and that neither mode of control is an indirect effect of the other.
This study is described in more detail in Sudarsanam P et al.(2000) Proc Natl Acad Sci U S A 97:3364-9
Keywords: other
Type: Expression profiling by array
Contributor: P,,Sudarsanam; V,R,Iyer; P,O,Brown; F Winston
Contact: Name: Stanford Microarray Database; Email: array@genome.stanford.edu; Phone: 650-498-6012; Department: Stanford University, School of Medicine; Institute: Stanford Microarray Database (SMD); Address: 300 Pasteur Drive; City: Stanford; State: CA; Zip/postal_code: 94305; Country: USA; Web_link: http://genome-www5.stanford.ed ..
Ftp SOFT: GSE21 SOFT
Ftp sMatrix: GSE21 sMatrix
   
 
 
Meltzerlab/GB/CCR/NCI/NIH @2008 Contact: e-mail
Powered by BxAF Search