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ID: 8
Geo Accession: GSE8 (GEO Site)
Title: Topoisomerase function in E. coli replication fork movement
Platform id: GPL16 GPL17
Sample id:
Total 21 GSM
Status: Public on Jul 30 2001
Submission date: 2001-07-30
Last update date: 2012-02-23
Pubmed id: 10944214  
Summary: Analysis of topoisomerase function in bacterial replication fork movement: use of DNA microarrays.
We used DNA microarrays of the Escherichia coli genome to trace the progression of chromosomal replication forks in synchronized cells. We found that both DNA gyrase and topoisomerase IV (topo IV) promote replication fork progression. When both enzymes were inhibited, the replication fork stopped rapidly. The elongation rate with topo IV alone was 1/3 of normal. Genetic data confirmed and extended these results. Inactivation of gyrase alone caused a slow stop of replication. Topo IV activity was sufficient to prevent accumulation of (+) supercoils in plasmid DNA in vivo, suggesting that topo IV can promote replication by removing (+) supercoils in front of the chromosomal fork.
This study is detailed in Khodursky AB et al.(2000) Proc Natl Acad Sci U S A 97:9419-24
Keywords: other
Type: Other
Contributor: A,B,Khodursky; B,J,Peter; M,B,Schmid; J DeRisi; D Botstein; P,O,Brown; N,R,Cozzarelli
Contact: Name: Stanford Microarray Database; Email: array@genome.stanford.edu; Phone: 650-498-6012; Department: Stanford University, School of Medicine; Institute: Stanford Microarray Database (SMD); Address: 300 Pasteur Drive; City: Stanford; State: CA; Zip/postal_code: 94305; Country: USA; Web_link: http://genome-www5.stanford.ed ..
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