# Meltzerlab GEO Microarray Tool - View GSM Details
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ID: 2
GEO Accession: GSM2 (GEO site )
Title: HCMV-infected foreskin fibroblasts
Series ID: GSE506
Platform ID: GPL4
Status: Public on Sep 28 2000
Submission date: 2000-09-28
Last update date: 2008-11-19
Type: SAGE
Channel count: 1
Source name ch1: mRNA of HCMV-infected human foreskin fibroblasts
Organism ch1: Homo sapiens
Molecule ch1: total RNA
Description: Primary human foreskin fibroblasts at passages 15-18, cultured in Eagles Minimal Essential Medium (MEM) supplemented with penicillin and 10% fetal calf serum (FCS).
Confluent cells serum starved for 3 days with MEM containing 1% FCS.
85% of cells in G0/G1 cell cycle phase.
Cells infected at a multiplicity of infection (m.o.i.) of 5 with human cytomegalovirus (HCMV) strain AD169 infectious particles (virions) for 90 min at 37┬░Celsius.
mRNA isolated using a direct mRNA extraction kit.
Keywords = human cytomegalovirus, AD169, virions, HCMV-infected foreskin fibroblasts
Contact: Name: Marc Kenzelmann; Email: m.kenzelmann@dkfz.de; Phone: +41 31 632 3251; Fax: +41 31 632 3550; Department: Molecular Genome Analysis; Institute: Institute of Medical Microbiology, University of Bern; Address: Friedbuhlstrasse 51; City: Bern; Zip/postal_code: 3010; Country: Switzerland
Data row count: 5626
Meltzerlab/GB/CCR/NCI/NIH @2008 Contact: e-mail
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