| GEO Accession:
||GSM2 (GEO site )
||HCMV-infected foreskin fibroblasts
| Series ID:
| Platform ID:
| Status: ||Public on Sep 28 2000 |
| Submission date: ||2000-09-28 |
| Last update date: ||2008-11-19 |
| Type: ||SAGE |
| Channel count: ||1 |
| Source name ch1: ||mRNA of HCMV-infected human foreskin fibroblasts |
| Organism ch1: ||Homo sapiens |
| Molecule ch1: ||total RNA |
| Description: || Primary human foreskin fibroblasts at passages 15-18, cultured in Eagles Minimal Essential Medium (MEM) supplemented with penicillin and 10% fetal calf serum (FCS). |
Confluent cells serum starved for 3 days with MEM containing 1% FCS.
85% of cells in G0/G1 cell cycle phase.
Cells infected at a multiplicity of infection (m.o.i.) of 5 with human cytomegalovirus (HCMV) strain AD169 infectious particles (virions) for 90 min at 37°Celsius.
mRNA isolated using a direct mRNA extraction kit.
Keywords = human cytomegalovirus, AD169, virions, HCMV-infected foreskin fibroblasts
| Contact: ||Name: Marc Kenzelmann; Email: firstname.lastname@example.org; Phone: +41 31 632 3251; Fax: +41 31 632 3550; Department: Molecular Genome Analysis; Institute: Institute of Medical Microbiology, University of Bern; Address: Friedbuhlstrasse 51; City: Bern; Zip/postal_code: 3010; Country: Switzerland |
| Data row count: ||5626 |